Article : Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell
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영문초록

Bone morphogenetic proteins (BMPs) belong to the transforming growth factor (TGF-b) superfamily and are involved in osteoblastic differentiation. The largest TGF-bsuperfamily subgroup shares genetic homology with human BMPs (hBMPs) and silkworm decapentaplegic (dpp). In addition, hBMPs are functionally interchangeable with Drosophiladpp. Bombyx mori dpp may induce bone formation in mammalian cells. To test this hypothesis, we synthesized the 1,285-base pairs cDNA of full-length B. mori dpp using total RNAs obtained from the fat body of 3-day-old of the 5th instar larvae and cloned the cDNA into the pCEP4 mammalian expression vector. Next, B. mori dpp was expressed in C3H10T1/2 cells. The target cells transfected with the pCEP4-Bm dpp plasmid showed biological functions similar to those of osteogenic differentiation induction growth factors such as hBMPs. We determined the relative mRNA expression rates of Runt-related transcription factor 2 (RUNX2), osterix, osteocalcin, and alkaline phosphatase (ALP) to validate the osteoblast-specific differentiation effects of B. mori dpp by performing quantitative real-time T-PCR. Interestingly, mRNA expression levels of the 3 marker genes except RUNX2, in cells expressing B. mori dpp were much higher than those in control cells and C3H10T1/2 cells transfected with pCEP4. These results suggested that B. mori dpp signaling regulates osterix expression during osteogenic differentiation via RUNX2-independent mechanisms.
  • 가격4,000
  • 페이지수7 페이지
  • 발행년2013
  • 학회명한국잠사학회
  • 저자( Seung Won Park ) , ( Tae Won Goo ) , ( Gwang Ho Choi ) , ( Seok Woo Kang ) , ( Sung Wan Kim ) , ( Seong Ryul Kim )
  • 파일형식아크로뱃 뷰어(pdf)
  • 자료번호#3985591
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