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반응
2.ELISA(Enzyme-Linked Immunosorbent Assay)
3.침강법
4.HA(hemagglutination)
5.HI(hemagglutination inhibition test)
6.Immunoblotting (Western Blot)
7.MAT (microplate agglutination)
8. 면역확산법
9.보체결합반응 실험
10.TAT (tube agglutination test)
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immunoblot.com/westblot.htm
http://www.elpisbio.com/html-sub/protocols/western%20blotting.htm
http://www.ajou.ac.kr/%7Ebiotech/study/western/western%20blot.htm
http://home.pusan.ac.kr/%7Emolbio99/western.htm 1. Introduction
2. Material & Methods
3. Results
4. Discussion
* ECL
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immunoblot.com/pcr.htm
http://ee.uos.ac.kr/~dhlee/ldh12.htm
http://bifido.net/HTML-data/clon/PCR의%20원리와%20응용.htm
http://cowboys.gsnu.ac.kr/hjkang/paper/paper-04.htm
http://plaza.snu.ac.kr/~ymchoi/bcl.html Ⅰ. 서 론
1. PCR(Polymerase Chain Reaction)
2. PCR기계의 종류
Ⅱ. 본 론
1. P
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immunoblot.com
http://www.komabiotech.com
http://www.bmskorea.co.kr
http://www.chinju.ac.kr/~hak31/down/DNA Replication.hwp
http://kimwootae.com.ne.kr/mb/dnareplication.htm
http://www.dongeui.ac.kr/%7Ecblee/lec05/bio05.html
http://www.knou.ac.kr/~bhkim/study52.htm 〈요약〉
〈본문〉
1. PCR (p
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Immunoblotting (=western blotting)
1) SDS-PAGE: size별로 분리
(꼬여 있는 것이 SDS와 heat로 인해 펴짐, charge density가 일정).
2) Transfer of membrane
그림 1. Protein transfer
3) Ponceau S staining: transfer buffer로 wash하면 없어짐
(destaining with transfer buffer)
4) Blocking: ant
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