5조 크로마티그래피 최종본

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5조 크로마티그래피 최종본에 대한 보고서 자료입니다.

목차

Ⅰ. Objective & Keyword

Ⅱ. Introduction & Background

Ⅲ. Apparatus

Ⅳ. Reagents

Ⅴ. Experimental process

VI. Caution & References

본문내용

Experimental process
Place the strips on a clean, dry sheet of paper and make a pencil mark about ¾” from one end of each strip. At the top of each strip, label the indicator that you will place on that strip.
(2) Dip the applicator into an indicator solution and put
a ¼” spot on the pencil line on the strip. Repeat this
procedure for each of the other indicators. Allow the
strips to dry.
(3) Pour about 50mL of the eluting solvent into two
250mL Erlenmeyer flasks. Close the flask by a stopper
immediately.
(4) When the sample spots are dry, hang the strips in a
flask, so that the ends of the strips, but not the
sample spots, are in the solvent.
(5) Let the solvent rise on the strip until the solvent front
has moved above the band from the indicator. At
that time, remove the strip from the flask. Draw a
pencil line along the solvent front of the strip, and
allow the strips to dry for several minutes.
(6) When the strips have dried, hold each strip for 2~3
minutes over the open mouth of 2~3 drops of
concentrated ammonia, and record the color of the
band associated with each indicator in this basic
environment.
(7) Pour about 10mL dimethylglyoxime into the Petri dish,
and immerse the strip in it. After it is completely wet,
take out and dry the strip. Draw a pencil line of
appeared spots. Repeat the 7th step for ammonium
thiocyanate solvent.
(8) When you know the position of the band to be
associated with each indicator, measure the distance
from the point at which the indicator was applied to
the center of the top of the band.
(9) For each indicator, also measure the distance
(10) Calculate Rf values for each indicator.

키워드

5조 크로마티그래피 최종본,   화학 공학 기초 실험,   Apparatus,   크로마티그래피