Lab practice_cell handling & RNA extraction

소개글

Lab practice_cell handling & RNA extraction에 대한 보고서 자료입니다.

목차

1. 실험의 목적
2. 실험 관련 이론내용
3. 실험 준비물 및 과정
4. 실험 결과
5. 실험에 대한 고찰 및 논의

본문내용

 Trizol : Phenol, a component of Trizol, plays a role in collapsing proteins and lipids in cells, and guanidine isothiocyanate is a powerful component that helps protein denaturation.
 Chloroform :
1. make protein denaturation as an organic solvent and cell membrane destruction
2. Use to remove phenol components (Trizol removed)
3. It's dense, so it sinks down and separates the layers (phase separation)
 Isopropanol : separates RNA combined with water and precipitates by combining Na+ and RNA contained in Trizol(The combination of Na+ and phosphate ions is easier in alcohols with lower polarity than in water)
 70 % ethanol : It washes Na+ combined with nucleic acid at a concentration suitable for washing out the salt (Roles of precipitating proteins and nucleic acids)
 Distilled water(D.W.) : It is a substance that does not have the ability to break down RNA and DNA (no RNase and DNase), so we preserve RNA using D.W..

키워드

바이오메디컬,   cell handling ,   RNA extraction,   report,   레포트