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<SIMS 활용분야>
1. 반도체산업: 반도체내의 impurity analysis, dopant의 distribution measurement, surface contamination measurement
2. 금속소재산업: 고순도재료의 극 미량 경원소농도 및 분포분석, 소재표면분석: (coating, adhesion, painting, corrosion, wear 등)
3. 정
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secondary and tertiary structure ( 11) X-ray calligraphy )
6. DNA detection ( 8) Dot-blot assays hybridization )
보기
1) Amino acid analysis 2) LAL-test 3) SDS-PAGE 4) HPLC 5) isoelectric ~ 6) peptide mapping 7) capillary electrophoresis 8) Dot-blot assays hybridization 9) Mass spectrometry 10) 2-D
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Mass spectrometry is an instrumental approach that allows for the mass measurement of molecules.
Mass spectrometer determines the molecular weight of chemical compounds by ionizing, separating, and measuring molecular ions according to their mass-to-charge ratio (m/z).
Mass spectrometers have be
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of proteins
Protein characterization
Introduction
Functional study
Evidence of purity
1. SDS-PAGE
2. Isoelectric focusing
3. two dimensional electrophoresis
4. capillary electrophoresis
5. HPLC
6. Mass spectrometry
Molecular mass determination : mass spectrometry
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아미노산 서열 결정방법
1) Polypeptide의 부분분해
2) N-말단 결정법
3) C-말단 결정법
4) Tandem mass spectrometry
7. 분자량 측정방법
1) DNA분자의 분자량 측정
2) 단백질의 분자량 측정
8. 생화학적 특성
9. 결론
10. 참고문헌과 싸이트
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mass spectrometry와 정확한 질량 분석(accurate mass determination)은 세 개의 disialylated species의 structure를 나타내었다:Hex4HexNAc4NeuAc2, Hex6HexNAc4NeuAc2, Hex6HexNAc5NeuAc2(fig.6)
현재까지 이것들이 식물에서는 발견되고 있지 않기 때문에 제시된 glycan 패턴은 시퀀싱
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