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Advantages
– Many kinds of cell lines
– Generally easy to grow and manipulate
– Proliferate indefinitely
Disadvantages
– Ploidy problems
– Loss of biochemical properties of parent tissue
Advantages
– Similar chromosome number as parent tissue
– Perf
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cells, although some may be sensitive to EDTA.
6. EDTA prewash, then 0.25% trypsin
with 1 mM EDTA
Strongly adherent cells, particularly epithelial and
some tumor cells (note; EDTA can be toxic to some cells)
7. 1 mM EDTA prewash, then 0.25% trypsin
and 200units/ml collagenase
Thick cultures, multila
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세포 내로 침입해 들어오는 바이러스와 같은 외부 DNA를 제거하기 위한 방어기전으로 가지고 있는 것이며, 자신의 DNA는 절단부위의 염기를 Methylation시켜서 자신의 제한효소가 자신의 DNA를 자를 수 없게 보호하고 있다. 이 효소는 보통 외부 DNA
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cultures
Streptomyces
mycelial in nature, which produces viscous cultures
algae
often require light for growth
animal cells
insect cells
fish cells
plant cells
slow-growing, delicate, often anchorage-dependent and with specific medium requirements
1.5. 보충교재
Asenjo, J. A. and Merchuk, J. C., "
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이루었다. 1. Microorganisms as Cells
2. Molecular Processes in Cells
3. Growth, Mutation, and Evolution
4. Cell Structure
6. Population, Communities, and Ecosystems
7. Laboratory Culture of Microorganisms
8. The Impact of Microorganisms on Human Affairs
9. A Brief History o
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