HPLC - Liquid Chromatography

소개글

HPLC - Liquid Chromatography에 대한 보고서 자료입니다.

목차

● What is LC?
● Column Chromatography
● What is HPLC?
● Components of HPLC
- Degasser
- Pump
- Injector
- Column
　·Affinity, Partition, SEC, IEX
- Detector
● Method Development

본문내용

Liquid Chromatography

Dionex Korea
Support Department





Chromatography
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　≪ 그 림 ≫

Chromatogram - Detector signal vs. retention time or volume

　≪ 그 래 프 ≫





What is LC?
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● A chromatographic technique in which the mobile phase is a liquid

● LC is a much older technique than GC, but was overshadowed by the rapid development of GC in the 1950’s and 1960’s

● LC is currently the dominate type of chromatography and is even replacing GC in its more traditional applications





Purpose of LC
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● Analytical - determine chemical composition of a sample

● Preparative - purify and collect one or more components of a sample





Problem:
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● What would be a good method for determining the following:
　- identity of accelerant at a suspected arson scene
　- amount of caffeine in Coca Cola
　- identifying active ingredient in an illicit drug preparation (LSD is heat sensitive)
　- purification and characterization of novel thermophilic plant enzyme from South America
　- identifying explosive materials used in Oklahoma bombing





Advantages of LC compared to GC
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● LC can be applied to the separation of any compound that is soluble in a liquid phase.

● LC more useful in the separation of biological compounds, synthetic or natural polymers, and inorganic compounds

● Liquid mobile phase allows LC to be used at lower temperatures than required by GC

● LC better suited than GC for separating compounds that may be thermally labile

● Most LC detectors are non-destructive

키워드

HPLC교육 ,   HPLC,   LC,   미생물,   미생물실험