Cellular Senescence Western blot 레포트

소개글

Cellular Senescence Western blot 레포트에 대한 보고서 자료입니다.

목차

Cellular Senescence Western blot
Ⅰ. 서론
Ⅱ.재료 및 방법
Ⅲ.결과
Ⅳ. 논의
Ⅴ.참고 문헌

본문내용

APDH에 대한 선행된 연구인 \"Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and its physiological relevance\"(Jung et al., 2015)에서는 GAPDH가 단순히 대사 효소가 아니라, 다양한 생리학적 과정에서 중요한 역할을 한다는 것을 보여주고 있다. RNA의 지속적인 합성과 감소를 제어하는 것으로 발견되었으며, 또한 세포 분열, 미토콘드리아 기능, 염증 반응 등의 생리학적 과정에서도 중요한 역할을 한다고 밝혔다. 이처럼 GAPDH는 안정적으로 널리 분포하고 있다.
Ⅴ.참고 문헌
·Bass et al., 2017, An overview of technical considerations for Western blotting applications to physiological research., Scandinavian journal of medicine & science in sports, pp 4-25.
·Meftahi, et al., 2021, Applications of western blot technique: From bench to bedside., Biochemistry and Molecular Biology Education, pp 509-517.
·Kobayashi, et al., 2019, Cell sheet tissue engineering: cell sheet preparation, harvesting/manipulation, and transplantation., Journal of biomedical materials research, pp.955-967.
·Prive et al, 2007, Detergents for the stabilization and crystallization of membrane proteins., Methods, pp.388-397.
·Hans J et al, 2009, LC-MS/MS as an alternative for SDS-PAGE in blue native analysis of protein complexes, Proteomics, pp.4221-4228.
·Brianna et al, 2017, Western Blot Techniques, Molecular profiling: Methods and protocols, pp.133-139.
·Gibbons et al., 2014, Western blot: Protein transfer overview., North American Journal of Medical Sciences, p.158.
·Esposito et al., 2012, Using a large area CMOS APS for direct chemiluminescence detection in Western blotting electrophoresis. Medical Imaging, Biomedical Applications in Molecular, Structural, and Functional Imaging., pp 292-298
·Titli et al, 2018, Significance of circulatory DPP4 activity in metabolic diseases, IUBMB life, pp.112-119
·Shamloo, et al. 2019, p21 in cancer research., Cancers, p.1178.
·Hara et al., 2006, GAPDH as a sensor of NO stress. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, pp. 502-509.
·Kim et al, 2019, Senotherapeutics: emerging strategy for healthy aging and age-related disease, BMB Rep, pp 47-55
·Stephane et al, 2019, Senescence-associated secretory phenotype and its regulation, Cytokine, pp.15-22
·Jung et al., 2015, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and its physiological relevance, Journal of Cellular Biochemistry, pp. 1861-1869

키워드

cellular Senescence,   western blot,   웨스턴블럿,   웨스턴,   웨스턴블럿 실험,   실험레포트,   단백질,   antibody,   secondary body,   Western blotting,   Immunoblot,   Proliferating cell,   Senescent cell