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CST IMMUNOFLUORESCENCE TROUBLESHOOTING GUIDE
Name: Choi Jee In
Email Address: cji-012@daum.net
Company/University Name: Seoul National University
Type of Microscope/Imaging System:
Excitation Source (specific lasers, mercury bulb, etc)
Briefly Describe Problem:
A. Antibody Information
1.Catalog number: #9860S
2. Lot number (printed on tube label): 0000
3.Dilution you are using:
4. Secondary Antibody (leave blank if using conjugated antibody):
5. Secondary Vendor/Catalog Number:
Fluorochrome (eg. FITC):
6. Secondary Dilution:
7. Have you successfully used this secondary antibody for immunofluorescence? [YES][NO]
B. Specimen
1. Type: [ICC/CELL LINE][PARAFFIN SECTION][FIXED FROZEN SECTION][FRESH FROZEN SECTION]
2. Specimen Source (cell line or tissue, species)?
3. How were the cells/tissues treated prior to fixation (ligands, inhibitors, etc.)?
Name: Choi Jee In
Email Address: cji-012@daum.net
Company/University Name: Seoul National University
Type of Microscope/Imaging System:
Excitation Source (specific lasers, mercury bulb, etc)
Briefly Describe Problem:
A. Antibody Information
1.Catalog number: #9860S
2. Lot number (printed on tube label): 0000
3.Dilution you are using:
4. Secondary Antibody (leave blank if using conjugated antibody):
5. Secondary Vendor/Catalog Number:
Fluorochrome (eg. FITC):
6. Secondary Dilution:
7. Have you successfully used this secondary antibody for immunofluorescence? [YES][NO]
B. Specimen
1. Type: [ICC/CELL LINE][PARAFFIN SECTION][FIXED FROZEN SECTION][FRESH FROZEN SECTION]
2. Specimen Source (cell line or tissue, species)?
3. How were the cells/tissues treated prior to fixation (ligands, inhibitors, etc.)?
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