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Hassalbalch equation
3) 몰농도
4) buffer를 만드는 법, 계산과정
5)실험도구 및 과정
2. ion-exchange column chromatography
1)column chromatography의 원리
2)resin
-anion exchanger
-cation exchanger
3)elution (용출)
4)positive control & negative control
5)실험과정 및 도구
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exchanger chromatography (음이온 교환수지법)
3주차 실험 - Cation exchanger chromatography (양이온 교환수지법)
4주차 실험 → HIC (hydrophobic interaction chromatography)
5주차 실험 - affinity chromatography
6주차 실험 - protein 정량 (by Lorry and Folin method at mg level)
7주차
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exchanger current in the case of the intracellular Ca2+ increase.
In summary, there presents VOCC, which contribute to the intracellular Ca2+ increase in the case of membrane depolarization, and intracellular Ca2+ store, which releases Ca2+ to the cytoplasmic side in the case of some agonists applic
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