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Purification of the PCR Product (Amplified from λ DNA)
3. GE of the PCR Product & the Purified PCR Product
4. RE-digestion of the Purified PCR Product and a Cloning Vector
5. Ligation of the Digested DNAs
6. Preparation of Competent Cells & Transformation
7. Culture of Recombinants & Miniprep o
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from ancient bone extracts., Nucleic Acids Res., pp 881-882
·S. S. Freitas, J. et al, 2008, Optimization of Isopropanol and Ammonium Sulfate Precipitation Steps in the Purification of Plasmid DNA, Biotechnology progress, 1179-1186
·Jinlin et al, 2006, Identification of a glycine-rich protein from th
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of thimidine kinase, which converts AZT to the active triphosphate form. Selective advantage for the E. coli must be provided in the natural environment, so that such plasmids are maintained in their host bacterium. An example is the great diversity, of plamids conferring b-lactam resistance found a
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of siRNA, B. mechanism of RNA-interference
RT-PCR(P169),
Southern blotting
Northern blotting
Western blotting
Tag결합단백질(P177)
Reocombinant protein 정제: His-Tag
Protein Purification
EGFP and GFP
Blotting ?
Principles of Western blotting
RNA interference (RNAi) technology
His-Tag
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plasmid만을 추출하는 구조적 기본원리에 의한 방법이다.
방법Bacterial culture tube에 LB(일반영양배지 Luria Bertani) broth(액체배지)와 antibiotic(항생제)가 포함되어있는 곳에 재조합된 bacteria의 single colony를 picking하여 접종한 후 배양한다 bacterial cells로
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