Mouse 백혈구 분리관찰 및 면역반응

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Mouse 백혈구 분리관찰 및 면역반응에 대한 보고서 자료입니다.

목차

1. Title

2. Date

3. Purpose

4. Introduction

5. Materials & Methods

6. Results

7.Conclusions

8.References

본문내용

◈ immunization – methods
1.Active immunization
Achieved by inoculation
1)With pathogens that induce immunity but do not cause disease or
2)With antigenic components from the pathogens
2.Passive immunization
Preformed antibodies are transferred to a recipient: occurs naturally by transfer of maternal antibodies across the placenta to the developing fetus.
◈ Designing vaccines for active immunization
-Live, attenuated vaccines
-Inactivated or “killed “ vaccines
-Subunit vaccines : purified macromolecules derived from pathogens (3 kinds – inactivated toxoids, capsular polysaccharides & recombinant protein antigens)
-Conjugate vaccines
-DNA vaccines
-Recombinant vector vaccines
◈ white cell series
-Cranulocytes : neutrophils, eosinophils, basophils
-Mononuclear leucocytes: lymphocytes, monocytes
-Polymorphonuclear leucocyte : PMN
-각 구조와 설명은 추가로 첨부하였다.
◈ Giemsa stain(Wright’s stain)
-Methylene blue and eosin 의 mixture. 보통 giemsa powder 로 준비함
-A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed to a freshly prepared 5% Giemsa stain solution for 20 -30 minutes (in emergencies 5-10 minutes in 105 solution can be used), then flushed with tap water and left to dry.

키워드

미생물학 실습,   백혈구,   수동면역과 능동면역,   Giemsa stain(Wright’s stain)