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한다. 이온 교환 크로마토그래피(Ion exchange chromatography),
소수성 크로마토그래피(Hydrophobic interaction chromatography),
친화성 크로마토그래피(affinity chromatography),
biospecific affinity chromatography,
membrane chromatography,
purification of recombinant proteins
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chromatography (음이온 교환수지법)
3주차 실험 - Cation exchanger chromatography (양이온 교환수지법)
4주차 실험 → HIC (hydrophobic interaction chromatography)
5주차 실험 - affinity chromatography
6주차 실험 - protein 정량 (by Lorry and Folin method at mg level)
7주차 실험
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크로마토그래피 법이며, 이외에도 흡착 크로마토그래피(adsorption chromato-graphy), 액-액 분배 크로마토그래피(liquid-liquid partition chromatography), 소수성 상호반응 크로마토그래피(hydrophobic interaction chromatography), 종이 크로마토그래피(paper chromatography),
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Hydrophobic interaction의 기본은
released H2O molecules들의 enhanced freedom 때문인 것이다.
“Nonpolar soluk molecules are driven together in H2O not primarily
because they have a high affinity for each other but because H2O
bonds together strongly to itself.
c- 검정곡선(calibration curve)
"A gene
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Hydrophobic interaction의 기본은
released H2O molecules들의 enhanced freedom 때문인 것이다.
“Nonpolar soluk molecules are driven together in H2O not primarily
because they have a high affinity for each other but because H2O
bonds together strongly to itself.
c- 검정곡선(calibration curve)
"A gene
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