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M의 NaCl 등 eluant buffer를 5bed volumes으로 흘려준다. 마지막으로 재활용하기 위하여 1M NaCl buffer로 regeneration 시켜준다.
5.Crystallization
Chromatography 통하여 target 물질인 α-Amylase를 추출하였는데 이를 Crystallization 과정을 통하여 분순물과 물을 제거하
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