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electric fields
▶ Liposome mediated or microinjection
▶Regeneration of Transgenic Plant
1. Plant tissue culture
ㆍ Totipotency : unique ability of isolated plant cells to regenerate into a whole plant
ㆍ Callus formation - form almost any plant tissue using plant growth regulators
( ex-cytokinin, a
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Gene Transfer
살아있는 세포들에 유전인자를 인도하기 위하여
7
Genetic Markers
형질변환되었던 세포들을 확인하기 위하여
8
Replica Plating *
복제 도금
우뭇가사리류 접시에 박테리아 식민지들의 정확한 문서들에 도착하기 위하여
9
PCR
DNA
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Expression Vector
Fermenter
Field inversion gel electrophoresis(FIGE)
Fluorescence in situ hybridization(FISH)
Foot printing
Gel electrophoresis
Gel retardation
Geminivirus
Gene (유전자)
Gene addition
Gene cloning
Gene mapping
Gene subtraction
Gene therapy (유전자 치료)
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Electric field mediated gene transfer. Biochem. Biophys. Res. Commun. 107, 584-587
Wright, C., A. Carver, D. Cottom, D. Reeves, A. Scott, P. Simons, I. Wilmut, I. Caner and A.Colman. 1991. High level expression of active human alpha-l-antitrypsin in the milk of transgenic sheep. Biotechnology 9:830.
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