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analysis of protein complexes, Proteomics, pp.4221-4228.
·Brianna et al, 2017, Western Blot Techniques, Molecular profiling: Methods and protocols, pp.133-139.
·Gibbons et al., 2014, Western blot: Protein transfer overview., North American Journal of Medical Sciences, p.158.
·Esposito et al., 2012,
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blot analysis를 통해, protein을 membrane에 옮긴 뒤 protein of interest인 alkaline phosphatase(AP)와 특이적으로 결합하는 antibody를 이용해서 단백질 혼합물로부터 AP를 검출 및 확인하고자 한다. I, Introduction
1) Western blot의 정의와 원리
2) Western blot에서 사
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Detection
IV. Discussion
1. Stacking gel과 resolving gel의 차이
2. Running buffer와 transfer buffer의 조성 차이 및 그 역할
3. TTBS에 Tween-20을 넣는 이유
4. Direct detection과 indirect detection
5. Analysis of Results
① Transfer (with Ponceau S staining)
② Detection
V. References
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-conjugate (1/1000 d), Rabbit(1/2000 d)
12. 1XPBST로 5분, R.T. Shaking(fast). (2 times)
13. Detection 제목
실험방법
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use of water than males.
V. PR strategy
As mentioned above in the background analysis, many people lack of awareness of water shortage. This is because people, especially women cannot feel the actual shortage of water during the daily life. Moreover, relatively cheaper water bills also cause people
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