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-conjugate (1/1000 d), Rabbit(1/2000 d)
12. 1XPBST로 5분, R.T. Shaking(fast). (2 times)
13. Detection 제목
실험방법
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analysis of protein complexes, Proteomics, pp.4221-4228.
·Brianna et al, 2017, Western Blot Techniques, Molecular profiling: Methods and protocols, pp.133-139.
·Gibbons et al., 2014, Western blot: Protein transfer overview., North American Journal of Medical Sciences, p.158.
·Esposito et al., 2012,
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western.htm
http://www.immunoblot.com/westblot.htm
http://www.elpisbio.com/html-sub/protocols/western%20blotting.htm
http://www.ajou.ac.kr/%7Ebiotech/study/western/western%20blot.htm
http://home.pusan.ac.kr/%7Emolbio99/western.htm 1. Introduction
2. Material & Methods
3. Results
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Western blot analysis를 통해, protein을 membrane에 옮긴 뒤 protein of interest인 alkaline phosphatase(AP)와 특이적으로 결합하는 antibody를 이용해서 단백질 혼합물로부터 AP를 검출 및 확인하고자 한다. I, Introduction
1) Western blot의 정의와 원리
2) Western blot에
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Detection
IV. Discussion
1. Stacking gel과 resolving gel의 차이
2. Running buffer와 transfer buffer의 조성 차이 및 그 역할
3. TTBS에 Tween-20을 넣는 이유
4. Direct detection과 indirect detection
5. Analysis of Results
① Transfer (with Ponceau S staining)
② Detection
V. References
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