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Title
2. Objective
3. Principle
4. Method
(1) Cell culture
(2) Virus infection
5. Result
6. Discussion
(1) 배지
(2) FBS(Fetal Bovine Serum) 소태아혈청
(3) Antibiotics
(4) PBS
(5) TE (Tripsin+EDTA)
7. References
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infection 시키기때문에, Transfection이라하며, Virus를 쓰지않을때도 Transfection이라한다.
① Transient expression assay
-넣어준 DNA가 DNA Integretion되지 않았을때, 유전자가 살아있을동안 Cytoplasm에서의 표현을 보는것.
② Stable expression assay
넣어준 DNA가 핵안
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infection
Vaccines are available for some viral diseases
Complex virus: bacterial virus (ex. Bacterio phage)
- Capsid(head) : polyhedral, 핵산 들어있다
- Sheath :helical, 수축하여 안에 있는 관이 세균의 cell표면 관통하여 DNA 삽입, Lysozyme 들어있어 cell wall을 용
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Personal History
NSVD at home(40wks/3.2kg)
1st baby / 1 sibling
Formula milk feeding
Vaccination: BCG(+), Hepatitis(+)
DTP/Polio(-), MMR(-)
Chickenpox(-) Chief complaint
Present illness
Past History
Family History
Personal History
Review of System
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