|
proliferation cytokines: IL-2, IL-4, IL-5
-differentiation cytokines: IL-2, IL-4, IL-5, IFN-γ, TFG-β
Ⅱ. Methods
1. cell preparation
※cell counting 방법
2. stimulator로 stimulation
3. capture antibody coating
4. blocking
5. sample과 standard dilution의 preparation
6. standard와 sample l
|
|
|
과정
5. T cell의 activation과 B cell의 isotype class switching
-proliferation cytokines와 differentiation cytokines
6. ELISA의 원리
7. ELISA의 종류
(1) Indirect ELISA
(2) Sandwich ELISA
(3) Competitive ELISA
(4) Modifications of ELISAs using biotin-streptavidin
(5) In-cell ELISAs
(6) ELI
|
|
|
proliferation/cytotoxicity assays are widely used in the field of drug screening and the investigating influences of cytokines, chemicals and growth factors on cell functions.
The reduction of tetrazolium salts is one of the reliable and common method to determine cell viability and cytotoxicity
|
|
메뉴펼치기
