새로운 인체 흉선 피질 상피세포 항원(DN4)의 생화학적 성질 및정상 조직에서의 표현

목차

새로운 인체 흉선 피질 상피세포 항원(DN4)의 생화학적 성질 및 정상 조직에서의 표현 분포 규명
목 차
I. 서론

II. 연구목적5

III. 연구재료 및 방법

IV. 연구결과

V. 고찰

VI. 결론

VII. 참고문헌

VIII. 영문초록
LIST OF TABLE

Table1 1. The distribution of DN4 antigen on human normal tissues determined by immunohistochemistry

Table 2. The distribution of DN4 antigen on normal hematopoietic cells determined by indirect immunofluoresce- nce and flowcytometric analysis

Table 3. The expression of DN4 on various human tumor cell lines

본문내용

, Monostori E, et al. Cortical thymocyte differentiation in thymomas: an immunohistologic analysis of the pathologic microenvironment. J Immunol. 1987, 138:687-98.
Tang JQ, Bene MC, Faur GC. CD9 recognizes an extracellular epitope of human keratin. In Knapp W (eds): Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford University Press, New York, 1989: 992-3.
Trapani JA, Browne KA, Dawson MJ, et al. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon- in myeloid cells. Immunogenetics. 1992, 36:369-76.
Vliet EV, Melis M, Ewijk WV: Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol. 1984, 14:524-9.
von Boehmer H: Thymic selection: a matter of life and death. Immunol Today. 1992, 13:454-8.
ABSTRACT
A novel cell surface molecule, DN4, defined by a monoclonal antibody raised against human thymic epithelial cell, showed a specificity for epithelial cells of the thymic cortex. This antigen was not expressed at detectable levels on any other types of tissues in the human body except for the thymus. Immunohistochemical analysis revealed that the reactivity of anti-DN4 monoclonal antibody(mAb) was restricted to the thymic cortex, and DN4 expressing cells were arranged in a reticular network with long processes between thymocytes. The cellular nature of DN4 positive cells was identified as cortical epithelial cells since DN4 was expressed in a subpopulation of freshly prepared thymic stromal cells which contain large amount of keratin, and the expression of DN4 were strictly confined to the cortical area within the thymus on immunohistochemical analysis of frozen tissues. Immunofluorescence and flow cytometric analysis revealed that a subpopulation of activated or interferon-γ treated monocytes were positive for DN4 whereas fresh prepared monocytes and PHA-activated lymphocytes were negative. So, DN4 may be a strong candidate for antivation marker of monocytes. Among the various human tumor cell lines tested, U937 and THP-1, which originate in monocytic lymphoma or leukemia, highly expressed DN4 molecule.
Immunopreciptitation of 125I-labeled cell lysates form THP-1 cell lines with anti-DN4 mAb yielded a single chain glycoprotein with an approximate size of 80-85kDa. There was a marked reduction in apparent molecular weight of approximately 40kDa in the immunoprecipitation of cell lysates after endoglycosidase F treatment. DN4, therefore, seemed to have a considerable amount of carbohydrate group.
DN4 appears to be a novel cortical epithelial cell antigen of the human thymus. Although the role of this molecule has not been well established experimentally, the possibility can be suggested that DN4 molecule might be involved in the positive selection of thymocytes which occurs predominantly in the thymic cortical area.
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Key words: Novel Glycoprotein, Cell surface molecule, Cortical epithelial cell, Human thymus

키워드

새로운 당단백,   세포 표면,   피질 상피세표,   인체 흉성