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, 2004, 세포노화, 세포자연사, 발암, Journal of The Korean Geriatrics Soci, pp1-2
·Casella G. et al, 2019, Cellular Senescence: Aging, Cancer, and Injury, Physiol Rev, 1047-1078 Cellular Senescence SA-β-gal staining
Ⅰ. 서론
Ⅱ.재료 및 방법
Ⅲ.결과
Ⅳ. 논의
Ⅴ.참고 문헌
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5.1 Bradford
5.2 UV spectrophotometry
6. SDS PAGE
6.1 PAGE gel 조성 및 준비과정
6.2 PAGE 만들기
6.3 Loading
6.5 Staining , Destaining , Dry
Ⅲ 결 과
1. Cell culture
2. Bradford
3. UV assay
4. SDS PAGE
Ⅳ 토 의
Ⅴ 참고문헌
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1) Cell thwaing and maintenance
2) Subculture & Transfection
3) Lac-Z staining
4) Significances of a gene in cells
5) MTT Assay
(2) 결과 및 분석
1) Clonal cell culture & subculture
2) Transfection
3) MTT Assay
Ⅲ. 결 론 (
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staining & viewing, photography
< A map of pGEM-3Z Vector > 실험목표
실험개요
I. PCR
II. Construction of Recombinant DNA
III. Transformation
IV. Characterization of Recombinants
실험내용
1. PCR of λDNA with LP primers & Gel Electrophoresis
2. Purification of the PCR Produc
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staining of the satellite regions of human acrocentric chromosomes. Experientia 31:260-262, 1975.
Latt SA. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Nat Acad Sci. US 70:3395-3399, 1973.
Sahastrabuddhe CG, Pathak S, Hsu TC. Responses of ma
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