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Transformation of E. coli XL-1 Blue and DM700 with pTrc99A/topAcysB DNA
http://www.bch.msu.edu/bchug/web/bch472/LM5A.html
2) New England Biolab - NEB-5α
http://www.neb.com/nebecomm/products/productC2988.asp
3) 네이버 지식사전 - amphicillin, lac operon
http://terms.naver.com/entry.nhn?docId=293243&nb
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of queensland diamantia institute
\" The Alkaline Lysis Mini-Plasmid Preparation\"
2. 위키백과 “에틸렌다이아민테트라아세트산“
3. QIAGEN \"QIAprep Spin Miniprep Kit\"
4. 네이버 지식사전 “흡광도, hyperchromic effect\" Introduction
-solution 1,2,3
-EDTA
-miniprep.
-흡광도(ab
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of the PCR Product (Amplified from λ DNA)
3. GE of the PCR Product & the Purified PCR Product
4. RE-digestion of the Purified PCR Product and a Cloning Vector
5. Ligation of the Digested DNAs
6. Preparation of Competent Cells & Transformation
7. Culture of Recombinants & Miniprep of Plasmid
8.
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of Plasmid DNA
Introduction : 플라스미드
DNA 클로닝: 공여체 DNA를 다량 복제하는 것
DNA 재조합 과정
Material
Subject Enzyme treatment
Introduction : 제한효소
Method
Result
Subject Ligation and Transformation
Introduction : transformation -형질전환
Transfo
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결과도 생각할 수 있다.
5. Reference
http://tong.nate.com/impresser/9173824
http://www.biologicalprocedures.com/bpo/arts/1/16/m16.htm
James D. Watson외 5명, 2004, Molecular Biology of the gene 5E, CSHL press, pp485~488 1. Introduction
2. Materials & Method
3. Result
4. Disscussion
5. Refe
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of Private Education and Relationship with Public Education in Korea
Ki-Chang Song** Professor, Sookmyung Women\'s University
This study was performed to analyze the growth trends of private education on the base of concept of private education and private education expense, to investigate the cause
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of Molecular Biology fourth edition // p42-43 denaturation
전문진 // 2003년 8월 20일 // 현대의 생물공학과 생명공학 // p 69-71 PCR의 기본 원리
Watson, Baker, Bell, Gann, Levine, Losik // 2010년 2월 18일 // 왓슨의 분자생물학 6판//p746-748
Watson, Baker, Bell, Gann, Levine, Losik // 20
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