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이해 - 남상욱, 권혁빈, 최선심
http://www.google.co.kr/imgres? Abstract
Introduction
Materials and method
protein Induction
PCR
Agarose gel
Result and discussion
참고문헌
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Protein)
3. IPTG(Isopropyl-β-D-Thiogalactopyranoside)
II. Materials and Method
1. Cultuer condition
2. Agarose gel
2.1. 이론적 배경
2. 2. 제조 방법
3. Southern hybridization
3.1. 이론적 배경
3.2. 실험 방법
4. Western hybridization
4.1.1. SDS Polyacrylamide Gel electrophoresis
4.1.2.
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Protein_disulfide-isomerase
11) http://www.ncbi.nlm.nih.gov/protein/P09103.2
12) http://web.expasy.org/cgi-bin/protparam/protparam 유전자 재조합 단백질 정제 및 분석
Ⅰ 서 론(이론)
1. Cell culture
2. Harvest
3. Protein purification
4. Dialysis
5. Bradford
6. SDS P
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Protein expression / SDS-Page / IPTG induction
->protein expression
translation(원핵세포의 번역) 전사된 mRNA는 세포질에서 번역되어 단백질로 전환된다. 원핵세포의 경우는 핵과 세포질의 구별이 없어서 전자와 동시에 번역될 수 있다.
1)시작단계 : Ribosome의
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protein and prevention of cerulein-induced pancreatitis by water-immersion stress in rats. Int. J. Biochem. 26:805-811, 1994
26.Otaka M, Okuyama A, Otani S, Jin M, Okayama A, Itoh S, Iwabuchi A, Sasahara H, Itoh H, Tashima Y, Komatsu M. and Masamune O, Differential induction of HSP60 and HSP72 by di
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induction of urease in helicobacter pylori. Infect. Immun. 70, 2846-2852.
19. Weeks, D.L., Sachs, G., 2001. Sites of pH regulation of the urea channel of Helicobacter pylori. Mol. Microbiol. 40, 1249-1259.
20. Weeks, D.L. Eskandari, S., Scott, D.R., Sachs, G., 2000. A H+-gated urea channel: the li
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