수 종의 cytokine 이 사람 치주인대 섬유아세포의prostaglandin E2 , leukotriene

목차

I. 서 론
II. 연구 방법
III. 연구 성적
IV. 총괄 및 고안
V. 결 론
참고 문헌

본문내용

expression and interleukin-6 protein secretion in cells isolated from normal human bone: regulation by interleukin-1, J. Bone Miner. Res., 6:1258-1294, 1991.
77.Collins, J.L., Daniel, J.W., Cederquist, R., Simmelink, J.W., Enlow, D.H.: Stimulation of bone development by mechanical stress and inhibition of leukotriene biosynthesis, J. Dent. Res., 66:328, 1987. (Abstract 1771)
78.Collins, J.L., Daniel, J.W., Cederquist, R., Enlow, D.H.: The role of leukotrienes in force induced bone development, J. Dent. Res., 66:328, 1987. (Abstract 1772)
- Abstact -
Effect of various cytokines on the production of prostaglandin E2, leukotriene B4 and collagenase in human periodontal ligament fibroblasts in vitro
Jung-Ho Kim, D.D.S., M.S.D.
Dept of Orthodontics, Graduate School, Seoul National University
(Directed by Prof. Cheong-Hoon Suhr, D.D.S., M.S.D., Ph.D.)
This experiment was designed to study possible roles of interleukin-1β, interleukin-6 and tumor necrosis factor-α in bone remodeling by measuring their effects on PGE2, LTB4 and collagenase production when they were administered to human periodontal ligament fibroblasts.
Human periodontal ligament fibroblasts were collected from first premolars extracted for orthodontic treatment. They were incubated in the environment of 37℃, 5% CO2, and 100% humidity. They were treated with 0.25% trypsin-EDTA solution and centrifuged. PDL cells in the fifth to seventh passage were used for the experiment. Cells were seeded onto the culture dishes and when they were successfully attached, human recombinant interleukin-1 , interleukin-6, and tumor necrosis factor- were administered, alone or in combination. They were incubated for 4 , 8 and 24 hours and the levels of PGE2 , LTB4 and collagenase released into the culture media were assessed by enzymeimmunoassay and collagenase activity assay.
The conclusions are as follows:
1. IL-1 and TNF- were very active in stimulating the production of PGE2 and collagenase by human periodontal ligament fibroblasts, while IL-6 increased LTB4 production.
2. IL-1 significantly increased PGE2 , but LTB4 production was not increased. IL-1 is thought to act mainly via the cyclooxygenase pathway of arachidonic acid metabolism .
3. IL-6 tended to inhibit IL-1 in the production of PGE2 and collagense whereas IL-6 and TNF- showed additive effect in the level of PGE2.
The above cytokines increased the release of at least one of PGE2, LTB4 and collagenase. It suggests that cytokines are involved in bone remodeling process by stimulating PDL fibroblasts to produce various bone-resorptive agents. The roles of cytokines in bone remodeling as a whole would need further study.
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Key words : IL-1 , IL-6, TNF- , human periodontal fibroblast,
bone remodeling
감사의 글
본 논문을 위해 시종 지도 편달하여 주신 서정훈 지도교수님께 진심으로 감사드리오며, 도움의 말씀과 성원을 보내 주신 양원식 교수님, 남동석 교수님, 장영일 교수님, 김진태 교수님, 이기수 교수님과 많은 도움을 주신 정종평 교수님, 그리고 교정학 교실원 여러분께 감사드립니다.
또한 지켜 보아 주신 부모님께도 감사드리고 내조를 아끼지 않은 아내와, 아들에게도 고마움을 표합니다.
저자 씀

키워드

치주인대,   골,   IEUKOTRIEN,   치주,   섬유아세포,   인대