파명란 효소 가수분해물 최적조건설정 및 생리활성

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파명란 효소 가수분해물 최적조건설정 및 생리활성에 대한 보고서 자료입니다.

목차

I. 서론
3

II. 재료 및 방법
5
1. 재료
5
가.
재료 및 시약
5
나.
Sample의 제조
5
2. 방법
6
가.
일반성분 분석
6
나.
항산화능
6
1)
DPPH radical scavenging activity
6
2)
Hydrogen peroxide radical scavenging activity
7
3)
Hydroxyl radical scavenging activity
7
다.
효소 저해능
8
1)
α-Glucosidase inhibitory activity
8
2)
β-Glucuronidase inhibitory activity
8
3)
Elastase inhibitory activity
9
라.
Amino acid composition and free amino acid
9
마.
관능검사
10

Ⅲ. 결과 및 고찰
11
가.
일반성분 분석
1
나.
항산화능
11
1)
DPPH radical scavenging activity
11
2)
Hydrogen peroxide radical scavenging activity
12
3)
Hydroxyl radical scavenging activity
13
다.
효소 저해능
14
1)
α-Glucosidase inhibitory activity
14
2)
β-Glucuronidase inhibitory activity
16
3)
Elastase inhibitory activity
17
라.
Amino acid composition and free amino acid
18
마.
관능검사
20

Ⅳ. 요약
22

Ⅴ. 참고문헌
23

본문내용

mentation. J. Korean Soc Food Sci Nutr 33(9), 1515~1521
Table 1. Proximate composition of Alaska pollack roe
Proximate composition
(%)
Moisture
70.5±0.34
Ash
8.1±0.14
Protein
13.8±0.08
Lipid
0.4±0.24
Carbohydrate
7.2±0.16
Total
100.0
Table 2. DPPH radical scavenging activities of water extracts
from Alaska pollack roe
Sample
Concentration (mg/mL)
Inhibitory activity (%)
Alaska pollack roe
2
19.9±1.1
3
35.9±3.1
4
46.9±2.5
5
52.9±1.2
6
68.3±2.2
IC50 (mg/mL)
4.5±0.2
α-Tocopherola (IC50)
19.9 ug/mL
Table 3. Hydrogen peroxide radical scavenging activities of
water extracts from Alaska pollack roe
Sample
Concentration (mg/mL)
Inhibitory activity (%)
Alaska pollack roe
0.2
10.5±1.3
0.6
28.7±0.5
1.0
40.6±0.4
1.4
48.6±0.2
1.8
62.0±2.2
IC50 (mg/mL)
1.4±0.2
α-Tocopherola (IC50)
14.9 ug/mL
Table 4. The contents of free amino acid of fermented
Compositional
amino acid
Content (%)
Aspartic acid
7.4
Threonine
4.7
Serine
5.7
Glutamic acid
15.6
Glycine
3.2
Alanine
10.2
Valine
5.4
Cystein
1.7
Methionine
1.9
Isoleucine
4.7
Leucine
8.7
Tyrosine
4.5
Phenylalanine
4.1
Lysine
6.8
Histidine
2.0
Arginine
5.2
Proline
8.2
Total
100.00
Table 5. Free amino acid composition
Compositional
amino acid
Content (%)
Aspartic acid
1.8
Threonine
1.2
Serine
1.7
Glutamic acid
78.2
Glycine
1.2
Alanine
2.7
Valine
1.4
Cystein
0.1
Methionine
0.8
Isoleucine
1.2
Leucine
2.3
Tyrosine
1.1
Phenylalanine
1.1
Lysine
2.3
Histidine
0.5
Arginine
1.5
Proline
0.9
Total
100.00
Table 6. Nucleotieds of alaska pollack roe
Nucleotides
(mMol/g)
ATP
9.76
ADP
10.07
AMP
8.77
IMP
1.48
Inosine
6.96
Hyporxantine
17.97
Table 7. Independent factors, and their coded and actual levels used in RSM studies for optimizing hydrolysis conditions of alaska pollack roe
Independent factor
Symbol
code levels
-1
+1
Enzyme/substrate(v/w,%)
x1
1
3
Substrate/water(Time)
x2
2
10
Reaction time
x3
0.5
6
Table 8. Actual level of independent variables along with the observed values for the response variable, degree of hydrolysis of Alaska pollack roe
Run
Enzyme/Substrate
(v/w,%)
Substrate/Water
(Time)
Time
(h)
Degree of hydrolysis (DH) (%)
X1
X2
X3
1
3
10
0.50
64.50
2
2
2
3.25
18.99
3
3
10
6.00
72.35
4
1
10
6.00
67.77
5
3
2
0.50
17.36
6
1
6
3.25
52.25
7
2
6
6.00
53.34
8
3
2
6.00
19.94
9
3
10
6.00
71.04
10
2
8
3.25
52.10
11
1
10
0.50
51.83
12
1
10
6.00
67.38
13
1
2
0.50
15.24
14
2
6
6.00
53.84
15
1
2
6.00
18.96
16
3
2
0.50
13.60
17
3
6
3.25
57.77
18
2
10
0.50
57.05
Table 9. ANOVA table for quadratic model of central composite design
Source
Sum of square
df
Mean square
F value
P value
Model
7726.44
9
858.49
74.00
＜0.0001*
x1
72.78
1
72.78
6.27
0.0367*
x2
6291.80
1
6291.80
542.36
＜0.0001*
x3
156.70
1
156.70
13.51
0.0063*
x1x2
28.35
1
28.35
2.44
0.1566
x2x3
266.21
1
266.21
22.95
0.0014*
x1x3
1.95
1
1.95
0.17
0.6923
x12
51.37
1
51.37
4.43
0.0685
x22
4.16
1
4.16
0.36
0.5659
x32
14.94
1
14.94
1.29
0.2893
Residual
92.81
8
11.60
Lack of fit
70.97
4
17.74
3.25
0.1400
Pure Error
21.83
4
5.46
Cor total
7819.25
17

키워드

파명란,   효소,   가수분해물