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한다. 원심분리기는 중심축을 기준으로 고속회전을 하기 때문에 무게를 대칭 되도록 사용하여야 떨림이 없다.
Reference
http://en.wikipedia.org/wiki/Beer%E2%80%93Lambert_law
http://en.wikipedia.org/wiki/Alkaline_phosphatase
http://en.wikipedia.org/wiki/enzyme_kinetics
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activity might occur with the secondary antibody, resulting in nonspecific signal.
-An extra incubation step is required in the procedure.
*Sandwich ELISA 장점
- 분석하기 전에 sample을 purify하지 않아도 된다.
- direct나 indirect방법에 비해서 2-5배 정도 민감하다.
- 시료 내 항원의
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차이
2. 생화학적 차이
3. 생리학적 중요성
C. Apoptosis Detection
1. Cytotoxicity Detection(LDH)
2. Annexin-V-FLUOS staining
3. Cell Death Detection ELISA
4. Apoptotic DNA 전기영동
5. DNA Extraction for Apoptosis of Intergen
6. Caspase 3 Activity Assay
7. In Situ Cell Death Detection
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of enzymology (1981), ed. by N.C. Price & L. Stevens, Chap.2
2. Protein purification: principles and practice (1994), 3rd ed. by Robert K. Scope, Chap.3
3. Biochemistry textbook, enzyme kinetics
4. Biochemical calculations (1975), 2nd ed. by Irwin H. Segel, Chap.5) 1. 실험제목
2. 실험일시
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of DNA need to be present.
Levelling off stage: The reaction slows as the DNA polymerase loses activity and as consumption of reagents such as dNTPs and primers causes them to become limiting.
Plateau. No more product accumulates due to exhaustion of reagents and enzyme.
≪ 사 진 ≫
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of human
chromosomes. Proc Natl Acad Sci USA 1988, 85:6622-6626.
2. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL,
Coviello GM, Wright WE, Weinrich SL, Shay JW: Specific
association of human telomerase activity with immortal cells and
cancer. Science 1994, 266:2011-2015.
3. Colgin LM, W
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and day care center, take them as an opportunity to check young children's movement education of our country at present and utilize them as a reference when revising Nurri Curriculum in the future. For the subject of analysis, total 318 activities of five activity forms related to movement activity,
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효소작용과 구조 .............................................9
2. 우레아제의 유전적특징 .............................................12
3. NixA와 니켈 수송 .............................................13
4. NikR의 조절기작 ....................
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